규칙적인 운동이 고혈압유발 흰쥐의 심근에서 Bcl-2발현 및 Apoptosis변화에 미치는 영향

초록

The present study was designed to examine the antihypertensive effects of regular exercise by suppressing apoptosis in myocardium of L-NAME induced hypertension rats. We measured systolic and diastolic blood pressure (BP), and performed Bcl-2 Immunohistochemistry and TUNEL for apoptosis in heart tissue. Sprague-Dawley (n=40) rats were divided into 4 groups as follows: (1) control group (n=10, Con), (2) hypertension group(n=10, L-Con) (3) hypertension+exercise group (n=10, L-Ex), (4) Exercise group (n=10, Ex). To induce hypertension we administrated L-NAME (50 mg/kg) for 5days per week during 8weeks. The regular exercise was performed in swim water bath for 30min after 1hr L-NAME administration. Both L-NAME treatment and swim were performed 5 days /week with duration of 30minutes for 8weeks. BP was measured once per week. Results showed that the BP was significantly increased (p<.001) from 1week after L-NAME treatment, but systolic BP of L-Ex(p<.001) and diastolic BP of Ex(p<.05) groups showed markedly decreased from 4weeks and 2weeks after exercise, respectively. Particulary, the Bcl-2 expression in L-Ex was markedly increased as compared with L-Con group, whereas L-Con group showed decreased Bcl-2 expression and increased apoptotic cell than other groups. These results suggest that regular exercise would induce anti-hypertensive effect by controling the BP and preventing the apoptosis through bcl-2 expression in myocardium of L-NAME treated rats. Thus, regular exercise may be helpful to improvement of hypertensive heart function.

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