Loss of Calreticulin Uncovers a Critical Role for Calcium in Regulating Cellular Lipid Homeostasis

  • Wang, Wen-An
  • Liu, Wen-Xin
  • Durnaoglu, Serpen
  • Lee, Sun-Kyung
  • Lian, Jihong
  • ... Ahnn, Joohong
  • 외 3명
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초록

A direct link between Ca2+ and lipid homeostasis has not been definitively demonstrated. In this study, we show that manipulation of ER Ca2+ causes the re-distribution of a portion of the intracellular unesterified cholesterol to a pool that is not available to the SCAP-SREBP complex. The SREBP processing pathway in ER Ca2+ depleted cells remained fully functional and responsive to changes in cellular cholesterol status but differed unexpectedly in basal activity. These findings establish the role of Ca2+ in determining the reference set-point for controlling cellular lipid homeostasis. We propose that ER Ca2+ status is an important determinant of the basal sensitivity of the sterol sensing mechanism inherent to the SREBP processing pathway.

키워드

ELEMENT-BINDING PROTEIN-2ENDOPLASMIC-RETICULUMCHOLESTEROL HOMEOSTASISER STRESSSREBPACTIVATIONINSIG-1PERTURBATIONCALCINEURINPROTEOLYSIS
제목
Loss of Calreticulin Uncovers a Critical Role for Calcium in Regulating Cellular Lipid Homeostasis
저자
Wang, Wen-AnLiu, Wen-XinDurnaoglu, SerpenLee, Sun-KyungLian, JihongLehner, RichardAhnn, JoohongAgellon, Luis B.Michalak, Marek
DOI
10.1038/s41598-017-05734-x
발행일
2017-07
유형
Article
저널명
Scientific Reports
7
1
페이지
1 ~ 15

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