Effects of Nerve growth factor on the organotypic hippocampal slice culture using MEA system

Kim, Do-Hyoung; Yang, Se-Ra; Ji, Yoon-Sang; Song, In-Ho; Kim, Sun I.; Park, Ji-Ho; Kim, In Young

doi:10.1109/NER.2009.5109265

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Effects of Nerve growth factor on the organotypic hippocampal slice culture using MEA system

Kim, Do-Hyoung;
Yang, Se-Ra;
Ji, Yoon-Sang;
Song, In-Ho;
Kim, Sun I.;
... Kim, In Young;
외 1명

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초록

The neurotrophic effects of Nerve growth factor (NGF) on basal forebrain cholinergic neurons are well established. However, its potential actions as a fast-acting neuromodulator and the related receptor are not as well understood. The aim of this study is to investigate NGF-induced spatiotemporal neuronal activity changes in cultured rat organotypic hippocampal slices (OHCs), using a multielectrode array (MEA) system. NGF treatment rapidly produced long-term potentiation (LTP) like field excitatory post-synaptic potentials (fEPSP). Even after removing NGF by washing, the LTP-like fEPSP was maintained for a long time. To investigate the role of tropomyosin receptor kinase (Trk) receptor in the neural activity changes in rat OHCs, we treated the TrkA receptor blocker k252a. In the presence of the TrkA receptor blocker k252a the NGF-induced the LTP-like fEPSP was abolished. Taken together, these results show that the NGF-induced LTP-like fEPSP in rat OHCs might be strongly related with the TrkA receptor, and that MEA might be a good tool to investigate the NGF-induced neuronal activity changes in rat OHCs.

키워드

Long-term potentiation (LTP); Multielectrode array (MEA); Nerve growth factor (NGF); TrkA (tropomyosin receptor kinase) receptor; Basal forebrain; Hippocampal slice; Long-term potentiation (LTP); Long-term potentiations; Multielectrode array (MEA); Multielectrode arrays; Nerve growth factor; Nerve growth factor (NGF); Neural activity; Neuronal activities; Neurotrophic; Post-synaptic potentials; Receptor kinase; TrkA (tropomyosin receptor kinase) receptor; Tropomyosin; Bioactivity; Peptides

제목: Effects of Nerve growth factor on the organotypic hippocampal slice culture using MEA system

저자: Kim, Do-Hyoung; Yang, Se-Ra; Ji, Yoon-Sang; Song, In-Ho; Kim, Sun I.; Park, Ji-Ho; Kim, In Young

DOI: 10.1109/NER.2009.5109265

발행일: 2009-04

유형: Conference Paper

저널명: 2009 4th International IEEE/EMBS Conference on Neural Engineering, NER '09

페이지: 187 ~ 190