Real-time motion analysis of euglena cells swimming in a microfluidic chip for environmental toxicity biosensing

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초록

We developed an automatic categorization algorithm, which distinguishes the motion of micro-algae cells real-time with image processing. The motion of the micro-algae cells, confined in a closed space of microchip (micro-aquarium), was captured, and converted to trace images. The traces were sectioned by rectangular shapes, and categorized to straightforward swimming or on-site rotation, by aspect ratio and filling factor of the rectangular. The automatic categorization enables to detect the abnormal motion of the cells evoked by test chemicals real-time, which is important for sensing the metabolic-disturbing substances quickly.

키워드

ChemotaxisEuglena gracilisMicro-organism cellsReal-time motion analysisAlgaeAlgorithmsAspect ratioBiochemistryImage processingAutomatic categorizationChemotaxisEnvironmental toxicityEuglena gracilisMicro aquariumsMicrofluidic chipReal-time motionRectangular shapesMotion analysis
제목
Real-time motion analysis of euglena cells swimming in a microfluidic chip for environmental toxicity biosensing
저자
Ozasa, KazunariWon, JuneSong, SimonMaeda, Mizuo
발행일
2015-10
유형
Conference Paper
저널명
MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
페이지
2002 ~ 2004