cP1P Maintains Long-Term Pluripotency in Human Pluripotent Stem Cells

초록

Long-term culture of human pluripotent stem cells (hPSCs) can lead to spontaneous mutations, genomic abnormalities, and alterations in gene expression, thereby compromising their self-renewal and pluripotency. Thus, optimizing the long-term culture conditions of hPSCs is crucial. In this study, we introduce O-cyclic phytosphingosine-1-phosphate (cP1P, Axceso Biopharma Co. Ltd.), a novel culture additive structurally analogous to S1P, which markedly enhances hPSC self-renewal and survival. Our results demonstrate that cP1P supplementation promotes long-term proliferation of hPSCs by upregulating pluripotency markers and maintaining their ability to differentiate into cell types derived from the three germ layers. Furthermore, RNA-seq analysis reveals that cP1P alleviates long-term culture-induced upregulation of apoptosis- and chordate embryonic development–related genes, while preventing the downregulation of stem cell maintenance pathways. Collectively, these findings suggest that cP1P effectively supports the proliferation, pluripotency, and differentiation potential of hPSCs during both short- and long-term cultures.

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