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In vitro generation of mature dopamine neurons by decreasing and delaying the expression of exogenous Nurr1
- Park, Chang-Hwan;
- Lim, Mi-Sun;
- Rhee, Yong-Hee;
- Yi, Sang-Hoon;
- Kim, Boe-Kyoung;
- ... Jung, Sung Jun;
- 외 3명
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18Citations
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18초록
Neural stem/progenitor cell (NSC/NPC) cultures can be a source of dopamine (DA) neurons for experimental and transplantation purposes. Nurr1, a steroid receptor transcription factor, can overcome the limitations associated with differentiation of cultured NPCs into DA neurons. However, forced Nurr1 expression in NPC cultures generates non-neuronal and/or immature DA cells. We show here that the Nurr1 level and period of expression crucially affect the differentiation and maturation of Nurr1-induced DA neurons. Mature DA neurons were generated by manipulating Nurr1 expression patterns to resemble those in the developing midbrain.
키워드
Parkinson's disease; Dopamine neurons; Nurr1; Neuronal maturation; Doxycycline inducible expression system; ORPHAN NUCLEAR RECEPTOR; NEURAL PROGENITOR CELLS; MESENCEPHALIC PRECURSORS; TYROSINE-HYDROXYLASE; DIFFERENTIATION; RAT; SURVIVAL
- 제목
- In vitro generation of mature dopamine neurons by decreasing and delaying the expression of exogenous Nurr1
- 저자
- Park, Chang-Hwan; Lim, Mi-Sun; Rhee, Yong-Hee; Yi, Sang-Hoon; Kim, Boe-Kyoung; Shim, Jae-Won; Kim, Yong Ho; Jung, Sung Jun; Lee, Sang-Hun
- 발행일
- 2012-07
- 유형
- Article
- 권
- 139
- 호
- 13
- 페이지
- 2447 ~ 2451