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Preparation of Dexamethasone-Based Cationic Liposome and Its Application to Gene Delivery In Vitro
- Kim, Tae-Hun;
- Yu, Gwang Sig;
- Choi, Hye;
- Shim, Young Jung;
- Lee, Minhyung;
- 외 1명
WEB OF SCIENCE
14SCOPUS
14초록
In this study, dexamethasone was conjugated to PAMAM dendrimer (generation 0) and its gene transfection efficiency was investigated. To make a liposomal solution for gene delivery, DOPE was used as a fusogenic helper lipid. In gel retardation assay, PAMAM-dexamethasone conjugate (PAM-Dex)/DOPE liposome/DNA complex was completely retarded at 8:1 N/P (nitrogen/phosphate) ratio. The physicochemical characteristics are studied by measuring the average size distribution and zeta-potential values of the complexes. In vitro transfection assay showed that the PAM-Dex/DOPE liposome/DNA complex displayed higher gene delivery efficiency compared to PAMAM/DNA complex. In addition, PAM-Dex/DOPE liposome showed the lowest toxicity compared to PAMAM, PEI 25 kD and Lipofectamine. These results indicate that PAM-Dex/DOPE liposome has a potential to be used as an efficient gene carrier for gene therapy.
키워드
- 제목
- Preparation of Dexamethasone-Based Cationic Liposome and Its Application to Gene Delivery In Vitro
- 저자
- Kim, Tae-Hun; Yu, Gwang Sig; Choi, Hye; Shim, Young Jung; Lee, Minhyung; Choi, Joon Sig
- 발행일
- 2011-02
- 유형
- Article; Proceedings Paper
- 권
- 11
- 호
- 2
- 페이지
- 1799 ~ 1802