Comparative Studies for Adhesion, Microcarrier, and Suspension Cell Culture of Hepatitis B Surface Antigen Expressing CHO-DG44 Cell Line

초록

Hepatitis B is a serious illness caused by hepatitis B virus (HBV). Hepatitis B vaccine, effective for prevention of HBV infection and its consequences, is administered more than one billion doses annually. Glycosylated forms of hepatitis B surface antigen (HBsAg) produced by CHO mammalian cell lines show better immune response than those from microorganisms. Improvements for production of HBsAg in mammalian cells will be necessary. The main procedure for HBsAg production is adhesion cell culture. Suspension cell culture is well known for its advantages, but few reports indicate cases of HBsAg production from adapted cells for suspension culture, none the less appearing lower productivity than adhesion cell culture. Comparative studies for adhesion, microcarrier, and suspension cell culture should therefore be beneficial. An adherent CHO-DG44 cell line expressing HBsAg was used for adhesion and microcarrier cell culture, the identical cell line was adapted for suspension cell culture. The production was observed in a serum-free media favorable for production. Observation of cell growth, substrate consumption, HBsAg production, and the intracellular content of HBsAg was made.