Remediation of petroleum hydrocarbon-contaminated sites by DNA diagnosis-based bioslurping technology

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초록

The application of effective remediation technologies can benefit from adequate preliminary testing, such as in lab-scale and Pilot-scale systems. Bioremediation technologies have demonstrated tremendous potential with regards to cost, but they cannot be used for all contaminated sites due to limitations in biological activity. The purpose of this study was to develop a DNA diagnostic method that reduces the time to select contaminated sites that are good candidates for bioremediation. We applied an oligonucleotide microarray method to detect and monitor genes that lead to aliphatic and aromatic degradation. Further, the bioremediation of a contaminated site, selected based on the results of the genetic diagnostic method, was achieved successfully by applying bioslurping in field tests. This gene-based diagnostic technique is a powerful tool to evaluate the potential for bioremediation in petroleum hydrocarbon contaminated soil.

키워드

BioslurpingBTEXDiagnosis technologyOligonucleotide microarrayTPHPOLYMERASE-CHAIN-REACTIONREAL-TIME PCRPSEUDOMONAS-PUTIDANUCLEOTIDE-SEQUENCEDEGRADING BACTERIAMICROBIAL-POPULATIONSPHENOL DEGRADATIONGENESMONOOXYGENASEDIVERSITY
제목
Remediation of petroleum hydrocarbon-contaminated sites by DNA diagnosis-based bioslurping technology
저자
Kim, SeungjinKrajmalnik-Brown, RosaKim, Jong-OhChung, Jinwook
DOI
10.1016/j.scitotenv.2014.08.002
발행일
2014-11
유형
Article
저널명
Science of the Total Environment
497-498
페이지
250 ~ 259