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초록
Induced pluripotent stem cell (iPSC) line HUi001-A was reprogrammed from skin fibroblasts via non-integrating, virus free self-replicating RNA. Skin fibroblasts from an 81-year-old female Caucasian familial Alzheimer's disease patient of Volga German family carrying N141I mutation in the PSEN2 gene (familial AD4, clinical summary confirmed Alzheimer's disease) was obtained from the Coriell Institute (AG09908). Generated iPSCs were characterized and pluripotency was confirmed.
키워드
alkaline phosphatase; brachyury protein; calvasculin; cell marker; hepatocyte nuclear factor 3beta; lin28 protein; messenger RNA; octamer transcription factor 4; presenilin 2; stage specific embryo antigen 3; stage specific embryo antigen 4; tbxt protein; tra 1 60 protein; tra 1 81 protein; transcription factor; transcription factor GATA 4; transcription factor NANOG; transcription factor PAX6; transcription factor Sox2; tuj1 protein; unclassified drug; uvomorulin; zfp42 protein; aged; Alzheimer disease; Article; Caucasian; cell differentiation; cell lineage; cell reprogramming technique; controlled study; ectoderm; endoderm; female; gene; H9 cell line; hui001 cell line; human; human cell; induced pluripotent stem cell; mesoderm; mRNA expression level; nuclear reprogramming; pluripotent stem cell line; point mutation; priority journal; puromycin resistance gene; RNA vector; skin fibroblast; very elderly
- 제목
- Generation of human induced pluripotent stem cell line from Alzheimer's disease patient with PSEN2 N141I mutation using integration-free non-viral method
- 저자
- Murti, Harry; Pieknell, Kelvin; Bachtiar, Indra; Sulistio, Yanuar Alan; Lee, Sang-Hun
- 발행일
- 2020-08
- 유형
- Editorial Material
- 권
- 47
- 페이지
- 1 ~ 5