모델 담즙산 나노입자 기반의 새로운 피부전달체

초록

Purpose: We assessed the in vivo efficacy of a cosmetic formulation comprising a transdermal delivery system of model bile acid nanoparticles after the development of a phytosphingosine-1-phosphate (PhS1P)/phytosterol (PS) carrier based on chemically modified lecithin with sodium deoxycholate, using cholesterol as a hydrophobic component. Methods: PhS1P/PS was loaded into a bile acidconjugated nanoparticle (PhS1P/PS-MBA nanoparticle) model via sonication. Franz diffusion cell experiment was performed to evaluate the skin penetration efficacy of the prepared nanoparticles and to comprehend the feasibility of applying the raw material. The nanoparticles were confirmed using a particle size analyzer, scanning electron microscope, and high-performance liquid chromatography system. In order to understand the effectiveness of the nanoparticles on the skin, we performed an in vivo test in human volunteers using the finished product. The elasticity, thickness, and dermal density of the applied skin were examined by using Cutometer (Courage+Khazaka electronic GmbH, Germany). Results: We confirmed through our tests that the nanoparticles could be successfully prepared with 85% yield and were approximately 120.3 nm in diameter. The product showed more efficient skin permeation than the control, thereby suggesting an easier uptake within the cells and a concomitant fast release of cleaved PhS1P/PS into the human skin. Safety assessment, elasticity, thickness, and dermal density of the nanoparticles on the human skin were examined to test the possible applicability of the nanoparticles to cosmetics. No adverse symptoms were observed. Conclusion: It is therefore recommended that cosmetic products should use PhS1P/PS-MBA nanoparticles in their formulation for easier absorbability into the skin. Our experiment confirms that this new material may have valuable influences on the human skin.

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