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Expansion of the prime editing modality with Cas9 from Francisella novicida
- Oh, Yeounsun;
- Lee, Wi-jae;
- Hur, Junho K.;
- Song, Woo Jeung;
- Lee, Youngjeon;
- 외 12명
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30초록
Prime editing can induce a desired base substitution, insertion, or deletion in a target gene using reverse transcriptase after nick formation by CRISPR nickase. In this study, we develop a technology that can be used to insert or replace external bases in the target DNA sequence by linking reverse transcriptase to the Francisella novicida Cas9, which is a CRISPR-Cas9 ortholog. Using FnCas9(H969A) nickase, the targeting limitation of existing Streptococcus pyogenes Cas9 nickase [SpCas9(H840A)]-based prime editing is dramatically extended, and accurate prime editing is induced specifically for the target genes in human cell lines.
키워드
Prime editing; Target expansion; CRISPR-Cas9; Ortholog; Francisella novicida; GENOMIC DNA; DUAL-RNA; BASE
- 제목
- Expansion of the prime editing modality with Cas9 from Francisella novicida
- 저자
- Oh, Yeounsun; Lee, Wi-jae; Hur, Junho K.; Song, Woo Jeung; Lee, Youngjeon; Kim, Hanseop; Gwon, Lee Wha; Kim, Young-Hyun; Park, Young-Ho; Kim, Chan Hyoung; Lim, Kyung-Seob; Song, Bong-Seok; Huh, Jae-Won; Kim, Sun-Uk; Jun, Bong-Hyun; Jung, Cheulhee; Lee, Seung Hwan
- 발행일
- 2022-04
- 유형
- Article
- 저널명
- Genome Biology
- 권
- 23
- 호
- 1
- 페이지
- 1 ~ 12