Expression of Ids and OUT mRNA in the preimplantation mouse embryos and uterus

초록

The helix-loop-helix (HLH) protein class of transcription factors are important regulators of cellular proliferation and differentiation in a number of cell types. The Id proteins are a family of related mammalian HLH protein, which are hypothesized to act negative regulator of DNA-binding bHLH proteins. We investigated into existence of Ids and OUT mRNA in the proimplantation mouse oocytes, embryos and uterus, uterus about estrus cycle. Id 1, 2 & OUT just existed in the blastocyst stage embryo, and Id 4 not existed in eggs. Id 3 expressed in GV stage oocyte, after 4-cell stage embryos. Expression of Ids and OUT mRNA in the reproductive cycles uterus analyzed that Id 1 entirely expressed in the reproductive cycle uterus, and Id 3 & OUT expressed in the other cycles uterus except the estrus cycle, and Id 4 slightly expressed in the others cycle except the diestrus cycle, but Id 2 only expressed in the metestrus cycle. Compared to the mouse implantation site and inter-implantation site, Id 1, 3 dramatic expressed, these genes increased each 1.79 fold, 1.75 fold in the implantation site. Id 2 was variable expressed in the samples. These results suggest that Id and OUT may act functinal regulator in the preimplantation mouse embryos and uterus and Ids and OUT may influence by sex hormone.