Alloxan에 의한 HIT-T15 세포의 산화적 손상에 대한 매실 (Prunus mume Sieb. et Zucc.) 주정추출물의 세포보호효과

초록

The present study was designed to examine the potential antidiabetic and antioxidant effect of ethanol extract from Prunus mume fruit (PME) against alloxan-induced oxidative stress in pancreatic -cells, HIT-T15. To evaluate the antidiabetic effect of PME, 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazoliu bromide (MTT) cell proliferation assay,lactate dehydrogenase (LDH) release assay, NAD +/NADH ratio and insulin secretion were assessed. We also measured its antioxidant effect against alloxan-induced oxidative stress in the cells by assessing the levels of the antioxidant enzymes including superoxide dismutase (SOD), glutathione S-transferase (GST), glutathione reductase (GR) and glutathione peroxidase (GPx). The results of this analysis showed that alloxan significantly decreased cell viability, increased LDH leakage, and lowered NAD +/NADH ratio and insulin secretion in HIT-T15 cells. However, PME significantly increased the viability of alloxan-treated cells and lowered LDH leakage. The intracellular NAD +/NADH ratio and insulin secretion were also increased by 1.5~1.9-fold and 1.4-fold, respectively, after treatment with the PME. The HIT-T15 cells treated with alloxan showed significant decreases in the activities of antioxidant enzymes, while PME significantly elevated the levels of antioxidant enzymes. Based on these results, we suggest that PME could have a protective effect against the cytotoxicity and dysfunction of pancreatic β-cells in the presence of alloxan-induced oxidative stress.

키워드