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Basic amino acids modified poly(Amidoamine) generation 2 for delivery of plasmid dna
- Lee, Young Ki;
- Thuy, Le Thi;
- Choi, Joon Sig;
- Lee, Minhyung
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0초록
Statement of Purpose: For past dacades, there has been numerous researches about developing non-viral vector for gene therapy applications. Among them, polymer vectors are attractive, due to its efficient synthesis and modification. This study demonstrates that modification of poly(amidoamine) generation 2 (PAMAM g2) with basic amino acids such as arginine and histidine was evaluated as a gene carrier, in terms of DNA transfection efficiency, cytotoxicity, serum tolerance and endosomal escape. Luciferase assay, MTT assay were performed to confirm the enhanced gene delivery abilities of polyplex. In addition confocal laser microscopy study was performed to visualize the efficient endosomal escape.
키워드
Gene therapy; Gene transfer; Basic amino acids; Confocal laser microscopy; DNA transfection; Efficient synthesis; Endosomal escapes; Luciferase assays; Nonviral vectors; Poly(amido amine); Amino acids
- 제목
- Basic amino acids modified poly(Amidoamine) generation 2 for delivery of plasmid dna
- 저자
- Lee, Young Ki; Thuy, Le Thi; Choi, Joon Sig; Lee, Minhyung
- 발행일
- 2019-04
- 유형
- Conference Paper
- 저널명
- Transactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium
- 권
- 40
- 페이지
- 546 ~ 546
- 언어
- ENG
- 발행국가
- 미국
- 분량
- 1 페이지
- ISSN
- P 1526-7547